Assimilation of nitrate by yeasts

Assimilation of nitrate by yeasts.

Nitrate assimilation has received much attention in filamentous fungi and plants but not so much in yeasts. Recently the availability of classical genetic and molecular biology tools for the yeast Hansenula polymorpha has allowed the advance of the study of this metabolic pathway in yeasts. The genes YNT1, YNR1 and YNI1, encoding respectively nitrate transport, nitrate reductase and nitrite red...

Determination of carbon assimilation patterns of yeasts by replica plating.

For the differentiation of species of yeasts, considerable value is attached to the ability of aimilating various carbon sources for growth. Beijerinck (1889) introduced for this general purpose the auxanographic plate technique which was adapted by Lodder (1934) for the study of sugar assimilation in the nonfermenting strains of the asporogenous yeasts. Diddens and Lodder (1942) expanded its u...

Nitrate assimilation in Chlamydomonas.

Assimilation of melezitose among various industrial yeasts

When we performed melezitose assimilation test in industrial yeasts, most sake yeasts failed to assimilate melezitose, while the other industrial yeasts could. We therefore tried to elucidate differences for melezitose assimilation among industrial yeasts. Results showed industrial yeasts had no melezitose resolving power on the cell surface, but had this in the cell. Also, the sake yeasts had ...

Rapid method for determining nitrate utilization by yeasts.

A test for the nitrate-reductase activity in yeasts has been developed, in which the reaction may be read after only 10 min of incubation. The rapidity of the test is due to the optimization of pH, substrate concentration, and temperature for the reaction.